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acrosomes  (Vector Laboratories)


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    Structured Review

    Vector Laboratories acrosomes
    Acrosomes, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhodamine+labeled+peanut+agglutinin/Rhodamine+labeled+Peanut+Agglutinin+(PNA)/pm42009655-454-29-32
    Average 94 stars, based on 250 article reviews
    acrosomes - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: MYADM binds human parechovirus 1 and is essential for viral entry
    Article Snippet: Similarly, GFP-positive cells were single-cell sorted and screened for knockout using Sanger sequencing with primers 5’-GCTGAAGCTCTCCACCTTCG-3’ and 5’-AGGTATCTGGGTGAGGCGTAG-3’ (IDT). .. The selected clone was further validated through Western blot analysis using an EXTL3-specific antibody (1:1000, Santa Cruz Biotechnology, clone G-5, sc-271986) and immunofluorescence staining using Rhodamine labeled Peanut agglutinin (Vector Laboratories, RL-1072) and an antibody against heparan sulfate (Amsbio, clone F58-10E4, 370255-S). ..

    Article Title: MYADM binds human parechovirus 1 and is essential for viral entry.
    Article Snippet: Similarly, GFP-positive cells were single-cell sorted and screened for knockout using Sanger sequencing with primers 5’-GCTGAAGCTCTCCACCTTCG-3’ and 5’- AGGTATCTGGGTGAGGCGTAG-3’ (IDT). .. The selected clone was further validated through Western blot analysis using an EXTL3-specific antibody (1:1000, Santa Cruz Biotechnology, cloneG-5, sc-271986) and immunofluorescence staining using Rhodamine labeled Peanut agglutinin (Vector Laboratories, RL-1072) and an antibody against heparan sulfate (Amsbio, clone F58-10E4, 370255-S). ..

    Immunofluorescence:

    Article Title: MYADM binds human parechovirus 1 and is essential for viral entry
    Article Snippet: Similarly, GFP-positive cells were single-cell sorted and screened for knockout using Sanger sequencing with primers 5’-GCTGAAGCTCTCCACCTTCG-3’ and 5’-AGGTATCTGGGTGAGGCGTAG-3’ (IDT). .. The selected clone was further validated through Western blot analysis using an EXTL3-specific antibody (1:1000, Santa Cruz Biotechnology, clone G-5, sc-271986) and immunofluorescence staining using Rhodamine labeled Peanut agglutinin (Vector Laboratories, RL-1072) and an antibody against heparan sulfate (Amsbio, clone F58-10E4, 370255-S). ..

    Article Title: MYADM binds human parechovirus 1 and is essential for viral entry.
    Article Snippet: Similarly, GFP-positive cells were single-cell sorted and screened for knockout using Sanger sequencing with primers 5’-GCTGAAGCTCTCCACCTTCG-3’ and 5’- AGGTATCTGGGTGAGGCGTAG-3’ (IDT). .. The selected clone was further validated through Western blot analysis using an EXTL3-specific antibody (1:1000, Santa Cruz Biotechnology, cloneG-5, sc-271986) and immunofluorescence staining using Rhodamine labeled Peanut agglutinin (Vector Laboratories, RL-1072) and an antibody against heparan sulfate (Amsbio, clone F58-10E4, 370255-S). ..

    Staining:

    Article Title: MYADM binds human parechovirus 1 and is essential for viral entry
    Article Snippet: Similarly, GFP-positive cells were single-cell sorted and screened for knockout using Sanger sequencing with primers 5’-GCTGAAGCTCTCCACCTTCG-3’ and 5’-AGGTATCTGGGTGAGGCGTAG-3’ (IDT). .. The selected clone was further validated through Western blot analysis using an EXTL3-specific antibody (1:1000, Santa Cruz Biotechnology, clone G-5, sc-271986) and immunofluorescence staining using Rhodamine labeled Peanut agglutinin (Vector Laboratories, RL-1072) and an antibody against heparan sulfate (Amsbio, clone F58-10E4, 370255-S). ..

    Article Title: MYADM binds human parechovirus 1 and is essential for viral entry.
    Article Snippet: Similarly, GFP-positive cells were single-cell sorted and screened for knockout using Sanger sequencing with primers 5’-GCTGAAGCTCTCCACCTTCG-3’ and 5’- AGGTATCTGGGTGAGGCGTAG-3’ (IDT). .. The selected clone was further validated through Western blot analysis using an EXTL3-specific antibody (1:1000, Santa Cruz Biotechnology, cloneG-5, sc-271986) and immunofluorescence staining using Rhodamine labeled Peanut agglutinin (Vector Laboratories, RL-1072) and an antibody against heparan sulfate (Amsbio, clone F58-10E4, 370255-S). ..

    Article Title: Expression and functional analyses of ephrin type-A receptor 2 in mouse spermatogonial stem cells
    Article Snippet: .. The sections (5 μm thickness) were prepared by staining them with rhodamine-labeled peanut agglutinin (Vector Laboratories, Burlingame, CA). ..

    Labeling:

    Article Title: MYADM binds human parechovirus 1 and is essential for viral entry
    Article Snippet: Similarly, GFP-positive cells were single-cell sorted and screened for knockout using Sanger sequencing with primers 5’-GCTGAAGCTCTCCACCTTCG-3’ and 5’-AGGTATCTGGGTGAGGCGTAG-3’ (IDT). .. The selected clone was further validated through Western blot analysis using an EXTL3-specific antibody (1:1000, Santa Cruz Biotechnology, clone G-5, sc-271986) and immunofluorescence staining using Rhodamine labeled Peanut agglutinin (Vector Laboratories, RL-1072) and an antibody against heparan sulfate (Amsbio, clone F58-10E4, 370255-S). ..

    Article Title: MYADM binds human parechovirus 1 and is essential for viral entry.
    Article Snippet: Similarly, GFP-positive cells were single-cell sorted and screened for knockout using Sanger sequencing with primers 5’-GCTGAAGCTCTCCACCTTCG-3’ and 5’- AGGTATCTGGGTGAGGCGTAG-3’ (IDT). .. The selected clone was further validated through Western blot analysis using an EXTL3-specific antibody (1:1000, Santa Cruz Biotechnology, cloneG-5, sc-271986) and immunofluorescence staining using Rhodamine labeled Peanut agglutinin (Vector Laboratories, RL-1072) and an antibody against heparan sulfate (Amsbio, clone F58-10E4, 370255-S). ..

    Article Title: Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa?
    Article Snippet: The nuclear DNA of cells was stained by Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA). .. The intactness of spermatozoa acrosomes was assessed by Rhodamine labeled Peanut Agglutinin (PNA-TRITC, Vector Laboratories Burlingame, CA, USA). ..

    Incubation:

    Article Title: TENT5C extends Odf1 poly(A) tail to sustain sperm morphogenesis and fertility.
    Article Snippet: To detect cell death, sections were further stained using the In Situ Cell Death Detection Kit (Roche, #11684817910) and incubated 1 hour at 37 °C with TUNEL reaction mixture (50 μL of enzyme solution mixed into 450 μL Label solution just prior to use) following the manufacturer’s instructions. .. Slides were then washed 3 times in PBS 1x-0.4 % triton X-100 and incubated for 1 hour at room temperature with secondary antibodies (1/1000), rhodamine-labeled peanut agglutinin to label acrosomes (PNA, 1/1000, Vector Laboratories, #RL-1072), and the Alexa Fluor® 647 anti-SYCP antibody (1/200, Abcam, #ab205847) to identify meiotic cells. .. After 3 washes in PBS 1x, the TrueView Autofluorescence Quenching Kit (Vector Laboratories, #SP-8400-15) was used to reduce cell autofluorescence.



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